• We next used the high content

  2018-10-24

  

  We next used the high-content imaging to further define hypertrophic pathway interactions in hESC-CMs and hiPSC-CMs and to compare this with data defining the phosphorylation effects of PE treatment. We have developed 2D and 3D imaging processes to analyze hypertrophic phenotype of our sodium ion ch

• Genetic manipulation by overexpression of exogenous factors

  2018-10-24

  

  Genetic manipulation by overexpression of exogenous factors such as Nanog, Klf2, and Prdm14 enables conversion of mouse EpiSCs to ESC-like cells (rESCs) (Gillich et al., 2012; Silva et al., 2009). Furthermore, transition of mouse EpiSCs to rESCs rarely occurs even after stimulation with LIF-STAT3 si

• br Materials and methods br Acknowledgments This work was

  2018-10-24

  

  Materials and methods Acknowledgments This work was supported by Spanish Ministry of Economy and Competitiveness and European Regional Development Fund (grant numbers SAF2013-43005-R and SAF2016-76004-R). The authors thank INDEPF (Instituto de investigación y desarrollo social de enfermedades

• RNA splicing occurs in spliceosomes which is

  2018-10-24

  

  RNA splicing occurs in spliceosomes, which is a complex consisting of RNA and proteins, including five small nuclear RNAs (snRNAs) (U1, U2, U4, U5, and U6 snRNA), hundreds of auxiliary protein factors, and small nuclear ribonucleoproteins (snRNPs) (Reed et al., 1988). The RNA splicing process is cat

• Here we report for the first time that the mouse

  2018-10-24

  

  Here, we report for the first time that the mouse eye possesses a primitive c-Kit+ cell that is self-renewing, clonogenic and multipotent, the three critical identifiers of tissue specific stem/progenitor bepridil (Weissman, 2000). In addition, lineage tracing techniques in vivo demonstrate that the

• The pig has many advantages over

  2018-10-24

  

  The pig has many advantages over mouse models for pre-clinical studies involving hESC-CPC transplantation, namely its similar heart size and physiology to human. In addition, pig cardiomyocytes have been shown to exhibit similar contraction rates and analogous action potential duration to humans (St

• br Experimental Procedures br Author Contributions br Acknow

  2018-10-24

  

  Experimental Procedures Author Contributions Acknowledgments We acknowledge S. Kobayakawa and M. Bialecka for discussions, S. Mendes and C. Visseren for technical support, M. Bouma for technical help with teratomas, and Z. Zhang for generation of mouse chimeras using equipment provided by I

• br Results br Discussion Analysis of DNA damage foci

  2018-10-24

  

  Results Discussion Analysis of DNA-damage foci in CHK1i-treated iib iiia inhibitors led to the remarkable discovery that CHK1i allowed FA-deficient iPSCs to escape G2 arrest and undergo mitosis without repairing the DSBs that had evidently caused their arrest. A previous study indicates that

• Recent studies indicate that the canonical Wnt pathway affec

  2018-10-24

  

  Recent studies indicate that the canonical Wnt pathway affects BMSC osteogenic differentiation. Wnt signaling can promote osteoblastic precursor differentiation into more differentiated osteoblasts and can serve as a negative regulator of adipogenesis (Glass et al., 2005; Krishnan et al., 2006). Thi

• br Results br Discussion WNT proteins in vivo are

  2018-10-24

  

  Results Discussion WNT proteins in vivo are often secreted locally and presented to responsive glycine transporter in a spatially controlled manner (Alexandre et al., 2014; Clevers et al., 2014; Farin et al., 2016; Goldstein et al., 2006; van den Heuvel et al., 1989). In this study, we present

• Theoretically enhanced induction of TRA

  2018-10-24

  

  Theoretically, enhanced induction of TRA1-60, a specific marker for reprogrammed iPSCs by miR-31 and shRNA-SDHA, can be achieved in two ways: improved reprogramming efficiency or inhibiting pariPSCs from differentiating back into somatic cells. We strongly favor the view that enhanced induction of T

• br Experimental Procedures br Author Contributions br

  2018-10-24

  

  Experimental Procedures Author Contributions Introduction Frontotemporal dementia (FTD) refers to a group of neurodegenerative diseases caused by focal but progressive neuronal loss, astrogliosis, and spongiosis in the frontal and temporal cortices associated with abnormal intracellular acc

• Previously it has been reported that

  2018-10-24

  

  Previously, it has been reported that iNPs can be directly converted from human or mouse FBs by TF combinations including all iPSC factor(s) (Lu et al., 2013), only certain factors (Wang et al., 2013; Yu et al., 2015), or single factors (Ring et al., 2012). Starting from a panel of 25 TFs, we identi

• One major drawback of current melanoma therapy is the

  2018-10-24

  

  One major drawback of current melanoma therapy is the development of resistance mechanisms against novel therapeutics targeting the MAPK members RAF or MEK. Upon MAPK inhibition, additionally acquired mutations reactivate the MAPK pathway by gain of RAF gene copy numbers (Shi et al., 2012; Villanuev

• The possibility of observing a new type of

  2018-10-24

  

  The possibility of observing a new type of quantum transport in an inelastic channel was predicted on the assumption that the motion of electrons occurs in such a manner that in addition to inelastic scattering, the electrons experience single scattering through a large angle. Later it was revealed

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